Premier Biosoft International has released v7.50 of AlleleID, which enables xMAP users to design differentiation assays. AlleleID employs a powerful algorithm to first align the sequences and then identify unique regions in each. The capture probes are located in these regions and then checked for multiplexing for use in bead-based suspension arrays, based on Luminex's xMAP technology.
In addition to the above, single template assays are also supported wherein oligos are designed for rapid and high-throughput SNP genotyping assays, particularly useful for population studies. It enables use of pre-designed proven oligos and designs a multiplex set compatible with them, saving costs. Included is a database of MicroPlex xTAGs (formerly known as FlexMAP TAGs) for automatic addition of appropriate tags to Allele Specific Primer Extension (ASPE) primers. Users also have the option to tag them on their own.
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